CD8 positivity in intratumoral lymphocytes

Clinical features

Patients’ age group, in years

Summary table
	Younger age	Older age
Mean	30.8	36.1
Standard deviation	51.8	57.4
Median	14.5	10.2
Interquartile range	24.2	38.8
Minimum	0.0	0.0
Maximum	291.0	243.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	624
P statistic	6.7e-01

Patients’ sex

Summary table
	Female	Male
Mean	31.7	33.4
Standard deviation	51.2	55.1
Median	7.0	14.0
Interquartile range	29.0	28.2
Minimum	0.0	0.0
Maximum	175.5	291.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	430
P statistic	4.7e-01

Patients’ race

Summary table
	Caucasian	African-American	Asian	Other
Mean	37.9	17.5	14	10.8
Standard deviation	59.5	21.5	NA	14.6
Median	13.8	7.5	14	5.0
Interquartile range	34.5	21.0	0	13.8
Minimum	0.0	0.5	14	0.0
Maximum	291.0	76.0	14	27.5

Kruskal-Wallis rank sum test
	Value
Statistic	2.152
Parameter	3
P statistic	0.54

History of other malignancies

Summary table
	No other tumor	Prostate cancer	Other tumor
Mean	33.8	38.9	22.9
Standard deviation	57.4	43.8	29.7
Median	13.5	30.0	10.0
Interquartile range	26.0	28.5	35.2
Minimum	0.0	5.5	0.0
Maximum	291.0	113.5	83.5

Kruskal-Wallis rank sum test
	Value
Statistic	1.289
Parameter	2
P statistic	0.52

Pathologic features

Tumor size group

Summary table
	Smaller size	Larger size
Mean	31.5	35.0
Standard deviation	62.6	43.9
Median	10.5	14.0
Interquartile range	28.4	50.0
Minimum	0.0	0.0
Maximum	291.0	175.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	472
P statistic	1.4e-01

Multifocality

Summary table
	Single tumor	Multifocal tumor
Mean	29.8	49.4
Standard deviation	50.1	76.3
Median	11.5	15.5
Interquartile range	27.0	25.5
Minimum	0.0	0.0
Maximum	291.0	243.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	202
P statistic	2.3e-01

Fuhrman grade

Summary table
	G1	G2	G3	G4
Mean	14.2	25.2	50.5	22.8
Standard deviation	18.7	34.7	79.1	25.1
Median	14.2	10.5	14.0	22.8
Interquartile range	13.2	29.0	42.5	17.8
Minimum	1.0	0.0	1.0	5.0
Maximum	27.5	157.0	291.0	40.5

Kruskal-Wallis rank sum test
	Value
Statistic	3.011
Parameter	3
P statistic	0.39

pT stage

Summary table
	pT1	pT2	pT3
Mean	33.5	29.0	32.6
Standard deviation	58.3	16.3	44.8
Median	10.5	29.0	13.5
Interquartile range	29.4	11.5	21.5
Minimum	0.0	17.5	4.0
Maximum	291.0	40.5	175.5

Kruskal-Wallis rank sum test
	Value
Statistic	2.131
Parameter	2
P statistic	0.34

Surgical margin

Summary table
	Negative margin	Positive margin
Mean	32.0	51.2
Standard deviation	54.4	52.9
Median	12.0	45.8
Interquartile range	27.5	73.8
Minimum	0.0	0.0
Maximum	291.0	113.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	102
P statistic	4.7e-01

Outcome features

Tumor progression

Summary table
	No tumor progression	Tumor progression
Mean	35.0	19.6
Standard deviation	56.6	26.0
Median	13.5	11.8
Interquartile range	32.0	14.8
Minimum	0.0	1.0
Maximum	291.0	79.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	270
P statistic	6.4e-01

Overall mortality

Summary table
	ANOD + AWD	DOC + DOD
Mean	36.8	14.0
Standard deviation	58.0	13.6
Median	13.8	11.5
Interquartile range	33.4	16.9
Minimum	0.0	0.0
Maximum	291.0	40.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	416
P statistic	2.9e-01

Cancer-related mortality

Summary table
	ANOD + AWD + DOC	DOD
Mean	33.3	20.8
Standard deviation	54.4	27.9
Median	13.5	20.8
Interquartile range	27.5	19.8
Minimum	0.0	1.0
Maximum	291.0	40.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	76
P statistic	7.9e-01

CD8–Ki67 positivity in intratumoral lymphocytes

Clinical features

Patients’ age group, in years

Summary table
	Younger age	Older age
Mean	0.8	1.0
Standard deviation	1.9	2.9
Median	0.0	0.0
Interquartile range	0.9	0.5
Minimum	0.0	0.0
Maximum	9.0	13.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	591
P statistic	9.7e-01

Patients’ sex

Summary table
	Female	Male
Mean	1.6	0.6
Standard deviation	3.6	1.7
Median	0.0	0.0
Interquartile range	0.8	0.2
Minimum	0.0	0.0
Maximum	13.5	9.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	541
P statistic	3.5e-01

Patients’ race

Summary table
	Caucasian	African-American	Asian	Other
Mean	1.0	0.7	0	0
Standard deviation	2.5	2.3	NA	0
Median	0.0	0.0	0	0
Interquartile range	1.0	0.0	0	0
Minimum	0.0	0.0	0	0
Maximum	13.5	8.0	0	0

Kruskal-Wallis rank sum test
	Value
Statistic	2.908
Parameter	3
P statistic	0.41

History of other malignancies

Summary table
	No other tumor	Prostate cancer	Other tumor
Mean	1.0	0.7	0.2
Standard deviation	2.6	1.1	0.4
Median	0.0	0.0	0.0
Interquartile range	0.5	1.0	0.5
Minimum	0.0	0.0	0.0
Maximum	13.5	2.5	1.0

Kruskal-Wallis rank sum test
	Value
Statistic	0.4427
Parameter	2
P statistic	0.8

Pathologic features

Tumor size group

Summary table
	Smaller size	Larger size
Mean	0.5	1.2
Standard deviation	1.6	3.0
Median	0.0	0.0
Interquartile range	0.5	0.5
Minimum	0.0	0.0
Maximum	9.0	13.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	580
P statistic	8.4e-01

Multifocality

Summary table
	Single tumor	Multifocal tumor
Mean	0.9	0.4
Standard deviation	2.5	0.6
Median	0.0	0.0
Interquartile range	0.1	1.0
Minimum	0.0	0.0
Maximum	13.5	1.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	228
P statistic	3.5e-01

Fuhrman grade

Summary table
	G1	G2	G3	G4
Mean	0	0.8	1.2	0
Standard deviation	0	1.9	3.3	0
Median	0	0.0	0.0	0
Interquartile range	0	0.9	0.5	0
Minimum	0	0.0	0.0	0
Maximum	0	8.0	13.5	0

Kruskal-Wallis rank sum test
	Value
Statistic	1.648
Parameter	3
P statistic	0.65

pT stage

Summary table
	pT1	pT2	pT3
Mean	0.7	0	1.4
Standard deviation	1.8	0	3.7
Median	0.0	0	0.0
Interquartile range	0.5	0	0.0
Minimum	0.0	0	0.0
Maximum	9.0	0	13.5

Kruskal-Wallis rank sum test
	Value
Statistic	0.8977
Parameter	2
P statistic	0.64

Surgical margin

Summary table
	Negative margin	Positive margin
Mean	0.7	2.6
Standard deviation	2.3	3.8
Median	0.0	1.2
Interquartile range	0.5	3.9
Minimum	0.0	0.0
Maximum	13.5	8.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	88
P statistic	1.8e-01

Outcome features

Tumor progression

Summary table
	No tumor progression	Tumor progression
Mean	1.0	0.2
Standard deviation	2.5	0.5
Median	0.0	0.0
Interquartile range	0.5	0.1
Minimum	0.0	0.0
Maximum	13.5	1.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	261
P statistic	7e-01

Overall mortality

Summary table
	ANOD + AWD	DOC + DOD
Mean	1.0	0.0
Standard deviation	2.6	0.1
Median	0.0	0.0
Interquartile range	1.0	0.0
Minimum	0.0	0.0
Maximum	13.5	0.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	441
P statistic	7e-02

Cancer-related mortality

Summary table
	ANOD + AWD + DOC	DOD
Mean	0.9	0
Standard deviation	2.4	0
Median	0.0	0
Interquartile range	0.5	0
Minimum	0.0	0
Maximum	13.5	0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	88
P statistic	3.9e-01

CD8 positivity in stromal lymphocytes

Clinical features

Patients’ age group, in years

Summary table
	Younger age	Older age
Mean	9.1	25.0
Standard deviation	15.5	68.3
Median	4.8	3.0
Interquartile range	8.5	8.2
Minimum	0.0	0.0
Maximum	69.0	230.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	153
P statistic	4.6e-01

Patients’ sex

Summary table
	Female	Male
Mean	12.8	14.6
Standard deviation	20.5	45.8
Median	8.5	3.0
Interquartile range	10.2	4.0
Minimum	0.0	0.0
Maximum	69.0	230.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	150
P statistic	3.6e-01

Patients’ race

Summary table
	Caucasian	African-American	Asian	Other
Mean	16.9	4.5	12	2.8
Standard deviation	45.2	5.2	NA	2.8
Median	4.0	3.0	12	1.5
Interquartile range	9.0	3.0	0	2.5
Minimum	0.0	0.0	12	1.0
Maximum	230.0	12.0	12	6.0

Kruskal-Wallis rank sum test
	Value
Statistic	1.364
Parameter	3
P statistic	0.71

History of other malignancies

Summary table
	No other tumor	Prostate cancer	Other tumor
Mean	8.9	1.5	58.2
Standard deviation	14.5	2.1	114.5
Median	4.5	1.5	1.5
Interquartile range	10.0	1.5	59.8
Minimum	0.0	0.0	0.0
Maximum	69.0	3.0	230.0

Kruskal-Wallis rank sum test
	Value
Statistic	1.925
Parameter	2
P statistic	0.38

Pathologic features

Tumor size group

Summary table
	Smaller size	Larger size
Mean	7.7	25.2
Standard deviation	14.9	62.7
Median	3.0	5.0
Interquartile range	8.0	11.0
Minimum	0.0	0.0
Maximum	69.0	230.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	120
P statistic	5.6e-01

Multifocality

Summary table
	Single tumor	Multifocal tumor
Mean	15.6	2.5
Standard deviation	42.2	4.4
Median	4.0	0.5
Interquartile range	10.5	3.0
Minimum	0.0	0.0
Maximum	230.0	9.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	90
P statistic	1.5e-01

Fuhrman grade

Summary table
	G1	G2	G3	G4
Mean	3.0	9.3	26.4	19
Standard deviation	4.2	16.3	71.6	NA
Median	3.0	3.0	4.0	19
Interquartile range	3.0	9.5	7.0	0
Minimum	0.0	0.0	0.0	19
Maximum	6.0	69.0	230.0	19

Kruskal-Wallis rank sum test
	Value
Statistic	2.295
Parameter	3
P statistic	0.51

pT stage

Summary table
	pT1	pT2	pT3
Mean	8.2	12.0	41.8
Standard deviation	15.2	9.9	92.3
Median	3.0	12.0	4.5
Interquartile range	7.2	7.0	10.8
Minimum	0.0	5.0	0.0
Maximum	69.0	19.0	230.0

Kruskal-Wallis rank sum test
	Value
Statistic	1.578
Parameter	2
P statistic	0.45

Surgical margin

Summary table
	Negative margin	Positive margin
Mean	14.8	1
Standard deviation	41.0	NA
Median	3.0	1
Interquartile range	10.0	0
Minimum	0.0	1
Maximum	230.0	1

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	24
P statistic	4.7e-01

Outcome features

Tumor progression

Summary table
	No tumor progression	Tumor progression
Mean	15.2	7.7
Standard deviation	43.0	5.9
Median	3.0	8.0
Interquartile range	7.2	8.0
Minimum	0.0	0.0
Maximum	230.0	14.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	58.5
P statistic	4.5e-01

Overall mortality

Summary table
	ANOD + AWD	DOC + DOD
Mean	15.5	7.3
Standard deviation	43.7	8.1
Median	3.0	6.5
Interquartile range	7.0	11.8
Minimum	0.0	0.0
Maximum	230.0	19.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	88
P statistic	9.8e-01

Cancer-related mortality

Summary table
	ANOD + AWD + DOC	DOD
Mean	14.0	19
Standard deviation	40.5	NA
Median	3.0	19
Interquartile range	7.8	0
Minimum	0.0	19
Maximum	230.0	19

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	3
P statistic	1.8e-01

CD8–Ki67 positivity in stromal lymphocytes

Clinical features

Patients’ age group, in years

Summary table
	Younger age	Older age
Mean	0	0
Standard deviation	0	0
Median	0	0
Interquartile range	0	0
Minimum	0	0
Maximum	0	0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	126
P statistic	NaN

Patients’ sex

Summary table
	Female	Male
Mean	0	0
Standard deviation	0	0
Median	0	0
Interquartile range	0	0
Minimum	0	0
Maximum	0	0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	120
P statistic	NaN

Patients’ race

Summary table
	Caucasian	African-American	Asian	Other
Mean	0	0	0	0
Standard deviation	0	0	NA	0
Median	0	0	0	0
Interquartile range	0	0	0	0
Minimum	0	0	0	0
Maximum	0	0	0	0

Kruskal-Wallis rank sum test
	Value
Statistic	NaN
Parameter	3
P statistic	NaN

History of other malignancies

Summary table
	No other tumor	Prostate cancer	Other tumor
Mean	0	0	0
Standard deviation	0	0	0
Median	0	0	0
Interquartile range	0	0	0
Minimum	0	0	0
Maximum	0	0	0

Kruskal-Wallis rank sum test
	Value
Statistic	NaN
Parameter	2
P statistic	NaN

Pathologic features

Tumor size group

Summary table
	Smaller size	Larger size
Mean	0	0
Standard deviation	0	0
Median	0	0
Interquartile range	0	0
Minimum	0	0
Maximum	0	0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	130
P statistic	NaN

Multifocality

Summary table
	Single tumor	Multifocal tumor
Mean	0	0
Standard deviation	0	0
Median	0	0
Interquartile range	0	0
Minimum	0	0
Maximum	0	0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	46.5
P statistic	NaN

Fuhrman grade

Summary table
	G1	G2	G3	G4
Mean	0	0	0	0
Standard deviation	0	0	0	NA
Median	0	0	0	0
Interquartile range	0	0	0	0
Minimum	0	0	0	0
Maximum	0	0	0	0

Kruskal-Wallis rank sum test
	Value
Statistic	NaN
Parameter	3
P statistic	NaN

pT stage

Summary table
	pT1	pT2	pT3
Mean	0	0	0
Standard deviation	0	0	0
Median	0	0	0
Interquartile range	0	0	0
Minimum	0	0	0
Maximum	0	0	0

Kruskal-Wallis rank sum test
	Value
Statistic	NaN
Parameter	2
P statistic	NaN

Surgical margin

Summary table
	Negative margin	Positive margin
Mean	0	0
Standard deviation	0	NA
Median	0	0
Interquartile range	0	0
Minimum	0	0
Maximum	0	0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	16
P statistic	NaN

Outcome features

Tumor progression

Summary table
	No tumor progression	Tumor progression
Mean	0	0
Standard deviation	0	0
Median	0	0
Interquartile range	0	0
Minimum	0	0
Maximum	0	0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	72.5
P statistic	NaN

Overall mortality

Summary table
	ANOD + AWD	DOC + DOD
Mean	0	0
Standard deviation	0	0
Median	0	0
Interquartile range	0	0
Minimum	0	0
Maximum	0	0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	84
P statistic	NaN

Cancer-related mortality

Summary table
	ANOD + AWD + DOC	DOD
Mean	0	0
Standard deviation	0	NA
Median	0	0
Interquartile range	0	0
Minimum	0	0
Maximum	0	0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	16.5
P statistic	NaN

Ki67 positivity in intratumoral lymphocytes

Clinical features

Patients’ age group, in years

Summary table
	Younger age	Older age
Mean	2.4	1.8
Standard deviation	2.9	1.9
Median	1.2	1.0
Interquartile range	1.6	0.5
Minimum	0.0	0.0
Maximum	15.0	8.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	604
P statistic	5.8e-01

Patients’ sex

Summary table
	Female	Male
Mean	2.4	2.0
Standard deviation	2.6	2.5
Median	1.0	1.0
Interquartile range	3.2	1.0
Minimum	0.0	0.0
Maximum	8.5	15.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	452
P statistic	9.8e-01

Patients’ race

Summary table
	Caucasian	African-American	Asian	Other
Mean	2.3	1.2	5.5	1.3
Standard deviation	2.7	1.3	NA	0.6
Median	1.0	1.0	5.5	1.0
Interquartile range	1.1	1.0	0.0	0.5
Minimum	0.0	0.0	5.5	1.0
Maximum	15.0	5.0	5.5	2.0

Kruskal-Wallis rank sum test
	Value
Statistic	5.738
Parameter	3
P statistic	0.13

History of other malignancies

Summary table
	No other tumor	Prostate cancer	Other tumor
Mean	2.2	1.5	2.1
Standard deviation	2.7	0.6	2.2
Median	1.0	1.5	1.0
Interquartile range	1.0	0.5	2.0
Minimum	0.0	0.5	0.0
Maximum	15.0	2.0	5.5

Kruskal-Wallis rank sum test
	Value
Statistic	0.3307
Parameter	2
P statistic	0.85

Pathologic features

Tumor size group

Summary table
	Smaller size	Larger size
Mean	1.8	2.5
Standard deviation	1.9	3.1
Median	1.0	1.0
Interquartile range	0.9	1.5
Minimum	0.0	0.0
Maximum	9.0	15.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	496
P statistic	4.1e-01

Multifocality

Summary table
	Single tumor	Multifocal tumor
Mean	2.2	1.6
Standard deviation	2.7	0.8
Median	1.0	1.5
Interquartile range	1.0	1.0
Minimum	0.0	0.5
Maximum	15.0	3.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	244
P statistic	7.5e-01

Fuhrman grade

Summary table
	G1	G2	G3	G4
Mean	1	1.6	3.2	1.2
Standard deviation	0	1.7	3.5	0.4
Median	1	1.0	1.5	1.2
Interquartile range	0	1.0	3.0	0.2
Minimum	1	0.0	0.0	1.0
Maximum	1	7.5	15.0	1.5

Kruskal-Wallis rank sum test
	Value
Statistic	6.194
Parameter	3
P statistic	0.1

pT stage

Summary table
	pT1	pT2	pT3
Mean	2.1	2.0	2.1
Standard deviation	2.7	0.7	2.2
Median	1.0	2.0	1.0
Interquartile range	1.0	0.5	1.0
Minimum	0.0	1.5	0.0
Maximum	15.0	2.5	8.5

Kruskal-Wallis rank sum test
	Value
Statistic	1.347
Parameter	2
P statistic	0.51

Surgical margin

Summary table
	Negative margin	Positive margin
Mean	2.1	2.0
Standard deviation	2.6	2.0
Median	1.0	1.2
Interquartile range	1.0	1.5
Minimum	0.0	0.5
Maximum	15.0	5.0

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	128
P statistic	9.7e-01

Outcome features

Tumor progression

Summary table
	No tumor progression	Tumor progression
Mean	2.2	1.9
Standard deviation	2.7	1.6
Median	1.0	1.5
Interquartile range	1.0	1.0
Minimum	0.0	0.5
Maximum	15.0	5.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	218
P statistic	7.3e-01

Overall mortality

Summary table
	ANOD + AWD	DOC + DOD
Mean	2.3	1.4
Standard deviation	2.7	1.4
Median	1.0	1.0
Interquartile range	1.1	0.6
Minimum	0.0	0.0
Maximum	15.0	5.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	410
P statistic	2.2e-01

Cancer-related mortality

Summary table
	ANOD + AWD + DOC	DOD
Mean	2.2	1.0
Standard deviation	2.6	0.7
Median	1.0	1.0
Interquartile range	1.0	0.5
Minimum	0.0	0.5
Maximum	15.0	1.5

Mann-Whitney U test for the sum of ranks
	Value
U-statistic	84.5
P statistic	5e-01